TLK1 Polyclonal antibody

TLK1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13564-1-AP

Synonyms

KIAA0137, PKU beta, TLK1, tousled like kinase 1

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Validation Data Gallery

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  WB analysis of HeLa using 13564-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13564-1-AP (TLK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13564-1-AP

  IP result of anti-TLK1 (IP:13564-1-AP, 4ug; Detection:13564-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IF Staining of HeLa using 13564-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13564-1-AP (TLK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13564-1-AP targets TLK1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLK1 fusion protein Ag4440
• Full Name: tousled-like kinase 1
• Calculated Molecular Weight: 766 aa, 87 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC032657
• Gene Symbol: TLK1
• Gene ID (NCBI): 9874
• RRID: AB_2203863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Neurosci; Circular RNA TLK1 aggravates neuronal injury and neurological deficits after ischemic stroke via miR-335-3p/TIPARP.; Authors - Fangfang Wu
human,mouse	WB,IHC	World J Gastroenterol; Tousled-like kinase 1 promotes gastric cancer progression by regulating the tumor growth factor-beta signaling pathway; Authors - Ruo-Chuan Sun ; KD Validated