CoraLite® Plus 647 Anti-Human TLR2 Rabbit Recombinant Antibody

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• CoraLite® Plus 647 Anti-Human TLR2 Rabbit Recombinant Antibody (CL647-98376-5)

TLR2 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL647-98376-5

Clone No.251864G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

• 

  FC experiment of human PBMCs using CL647-98376-5

  1x10^6 human PBMCs were surface stained with FcZero-rAb® CoraLite® Plus 405 Anti-Human CD14, and 0.25 ug CoraLite® Plus 647 Anti-Human TLR2 Rabbit RecAb (CL647-98376-5, Clone: 251864G3) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL647-98376-5

  1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human TLR2 Rabbit RecAb (CL647-98376-5, Clone: 251864G3) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98376-5 targets TLR2 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  DNA

• Full Name: toll-like receptor 2
• Calculated Molecular Weight: 784 aa, 90 kDa
• GenBank Accession Number: BC033756
• Gene Symbol: TLR2
• Gene ID (NCBI): 7097
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60603
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).