|Positive WB detected in
|mouse lung tissue, Jurkat cells
|Positive IHC detected in
|human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|RAW 264.7 cells
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:20-1:200
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17232-1-AP targets TLR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|TLR7 fusion protein Ag11073
|toll-like receptor 7
|Calculated molecular weight
|1049 aa, 121 kDa
|Observed molecular weight
|121 kDa, 100 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
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