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  • KD/KO Validated

TLR7 Polyclonal antibody

TLR7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17232-1-AP

Synonyms

TLR7, toll like receptor 7



Tested Applications

Positive WB detected inmouse lung tissue, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17232-1-AP targets TLR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR7 fusion protein Ag11073
Full Name toll-like receptor 7
Calculated molecular weight 1049 aa, 121 kDa
Observed molecular weight 121 kDa, 100 kDa
GenBank accession numberBC033651
Gene symbol TLR7
Gene ID (NCBI) 51284
RRIDAB_2203464
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TLR7 antibody 17232-1-APDownload protocol
IHC protocol for TLR7 antibody 17232-1-APDownload protocol
IF protocol for TLR7 antibody 17232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase

Authors - Gang Liu
ratWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
humanWB

Stem Cell Res Ther

Exosomal SNHG16 secreted by CSCs promotes glioma development via TLR7.

Authors - Ruijie Zhang
  • KD Validated
mouseWB,IF

Mol Neurobiol

Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment

Authors - Liyun Deng
mouseWB

Front Immunol

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations.

Authors - Lujie Yang
ratWB

Mol Cell Endocrinol

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Authors - Dongmin Li