TLR8 Antibody

TLR8 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B6A4

Cat no : 67317-1-Ig

Synonyms

CD288, TLR8, toll like receptor 8



Tested Applications

Positive WB detected inDaudi cells, U-937 cells, K-562 cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67317-1-Ig targets TLR8 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR8 fusion protein Ag16566
Full Name toll-like receptor 8
Calculated molecular weight 1041aa,120 kDa
Observed molecular weight 120 kDa
GenBank accession number BC101075
Gene symbol TLR8
Gene ID (NCBI) 51311
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses(PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms ,120 and 122 kDa.

Protocols

Product Specific Protocols
WB protocol for TLR8 antibody 67317-1-IgDownload protocol
IHC protocol for TLR8 antibody 67317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols