CoraLite®488-conjugated TLR8 Monoclonal antibody

TLR8 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3B6A4

Cat no : CL488-67317

Synonyms

CD288, TLR8, toll like receptor 8



Tested Applications

Positive IF detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 67317-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67317 targets TLR8 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLR8 fusion protein Ag16566
Full Name toll-like receptor 8
Calculated molecular weight 1041 aa, 120 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC101075
Gene symbol TLR8
Gene ID (NCBI) 51311
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.

Protocols

Product Specific Protocols
IF protocol for CL488 TLR8 antibody CL488-67317Download protocol
Standard Protocols
Click here to view our Standard Protocols