TMC1 Polyclonal antibody

TMC1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 20718-1-AP

Synonyms

Transmembrane cochlear-expressed protein 1, Transmembrane channel-like protein 1, transmembrane channel like 1, DFNB7, DFNB11

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Validation Data Gallery

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  WB analysis of mouse brain using 20718-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20718-1-AP (TMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 20718-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20718-1-AP (TMC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 20718-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20718-1-AP (TMC1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse brain using 20718-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TMC1 antibody (20718-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20718-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TMC1 antibody (20718-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20718-1-AP targets TMC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: transmembrane channel-like 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: NM_138691
• Gene Symbol: TMC1
• Gene ID (NCBI): 117531
• RRID: AB_10693676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane channel-like protein 1 (TMC1), is an integral membrane protein with six transmembrane domains and cytoplasmically oriented N- and C- termini (PMID: 20672865). Human TMC1 and mouse Tmc1 mRNA expression were detected in human fetal cochlea and inner-ear tissue of postnatal mouse, respectively. TMC1 is required for cochlear hair-cell function (PMID: 11850618). It is a component of hair cell transduction channels and contributes to permeation properties (PMID: 23871232). Mutations in TMC1 gene can cause progressive postlingual hearing loss and profound prelingual deafness (PMID: 11850618). The antibody is specific to TMC1.