TMEM120A Polyclonal antibody, PBS Only

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• TMEM120A Polyclonal antibody, PBS Only (17455-1-PBS)

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TMEM120A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 17455-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Ion channel TACAN, NET29, TACAN, TMPIT, Transmembrane protein 120A

• 

  WB analysis using 17455-1-AP (same clone as 17455-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17455-1-AP (TMEM120A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17455-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 17455-1-AP (same clone as 17455-1-PBS)

  WB result of TMEM120A antibody (17455-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM120A transfected HEK-293 cells. This data was developed using the same antibody clone with 17455-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17455-1-AP (same clone as 17455-1-PBS)

  Immunofluorescent analysis of HeLa cells using 17455-1-AP (TMEM120A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17455-1-PBS in a different storage buffer formulation.

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Product Information

17455-1-PBS targets TMEM120A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10849

  Product name: Recombinant human TMEM120A protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-322 aa of BC029487

  Sequence: MQPPPPGPLGDCLRDWEDLQQDFQNIQETHRLYRLKLEELTKLQNNCTSSITRQKKRLQELALALKKCKPSLPAEAEGAAQELENRMKERQGLFFDMEAYLPKKNGLYLSLVLGNVNVTLLSKQAKFAYKDEYEKFKLYLTIILILISFTCRFLLNSRVTDAAFNFLLVWYYCTLTIRESILINNGSRWAGRALREGSMEWGARTLREAGTGAGGDGGSLLQDQRLVGVPSLRVHLPVGSHADVVRRSHVPEIPEPIPLLFHVPELRAVSPVLLPERLPLPPAGAGRAAHHGPHCGGLPVLDVAGPHLPAAFSFLWTLLAAF

• Full Name: transmembrane protein 120A
• Calculated Molecular Weight: 322 aa, 36 kDa, 41 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC029487
• Gene Symbol: TMEM120A
• Gene ID (NCBI): 83862
• RRID: AB_2204630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXJ8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TMEM120A (also known as TACAN) is a nuclear envelope transmembrane protein essential for adipocyte differentiation and lipid metabolism. Originally identified through proteomic screening, it regulates spatial genome organization by repositioning pro-adipogenic genes from the nuclear periphery to the interior during adipogenesis. Adipocyte-specific knockout mice exhibit lipodystrophy with insulin resistance. TMEM120A was initially proposed as a mechanosensitive ion channel involved in mechanical pain sensing; however, subsequent structural studies revealed it contains a coenzyme A-binding site and shares homology with fatty acid elongases, challenging its role as an ion channel (PMID: 26024229; 34409941; 34374645).