TMEM165 Polyclonal antibody

TMEM165 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 20485-1-AP

Synonyms

TMEM165, TMPT27, TPARL, transmembrane protein 165, Transmembrane protein PT27, Transmembrane protein TPARL

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Validation Data Gallery

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  WB analysis of Jurkat using 20485-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20485-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20485-1-AP

  IP result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 1750 ug.

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  IF Staining of HepG2 using 20485-1-AP

  Immunofluorescent analysis of HepG2 cells using 20485-1-AP (TMEM165 antibody) at dilution of 1:100 and Rhodamine-labeled goat anti-rabbit IgG.

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  IF Staining of HepG2 using 20485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (20485-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Jurkat using 20485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

20485-1-AP targets TMEM165 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM165 fusion protein Ag14362
• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• RRID: AB_10695184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga
	WB	J Biol Chem; The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells.; Authors - Jiri Stribny
human	WB	Oncol Rep; TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity.; Authors - Jee-San Lee
mouse	WB	Biochem Biophys Res Commun; The Ca(2+)/H(+) antiporter TMEM165 expression, localization in the developing, lactating and involuting mammary gland parallels the secretory pathway Ca2+ATPase (SPCA1).; Authors - Timothy A Reinhardt
human	IHC	J Hepatocell Carcinoma; Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma; Authors - Shusheng Lin