TMEM165 Recombinant monoclonal antibody

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  IF Staining of HepG2 using 83146-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMEM165 antibody (83146-1-RR, Clone: 230517B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83146-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TMEM165 Recombinant antibody (83146-1-RR, Clone:230517B1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83146-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83146-1-RR against Human TMEM165 were performed. The affinity constant is below 1 pM.

TMEM165 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83146-1-RR

Clone No.230517B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

230517B1, Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83146-1-RR targets TMEM165 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14362

  Product name: Recombinant human TMEM165 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 61-177 aa of BC003545

  Sequence: VSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHC

• Full Name: transmembrane protein 165
• Calculated Molecular Weight: 324 aa, 35 kDa
• GenBank Accession Number: BC003545
• Gene Symbol: TMEM165
• Gene ID (NCBI): 55858
• RRID: AB_3670844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HC07
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM165 is a putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. It mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase, and provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway (PMID:22683087, 23569283, 27008884, 32047108). TMEM165 has two isoforms, with molecular weights of 28 kDa and 35 kDa respectively. It is also subject to ubiquitination modification, which may cause an increase in its molecular weight.