TMEM182 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 25366-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25366-1-AP (TMEM182 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25366-1-AP (TMEM182 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25366-1-AP (TMEM182 Antibody) at dilution of 1:50 (under 40x lens).

TMEM182 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25366-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Transmembrane protein 182, UNQ6974/PRO21957

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25366-1-AP targets TMEM182 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21906

  Product name: Recombinant human TMEM182 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 27-115 aa of BC130308

  Sequence: SDYWLLATEVGRCSGEKNIENVTFHHEGFFWRCWFNGIVEENDSNIWKFWYTNQPPSKNCTHAYLSPYPFMRGEHNSTSYDSAVIYRGF

• Full Name: transmembrane protein 182
• Calculated Molecular Weight: 229 aa, 26 kDa
• Observed Molecular Weight: 15 kDa 26 kDa
• GenBank Accession Number: BC130308
• Gene Symbol: TMEM182
• Gene ID (NCBI): 130827
• RRID: AB_2880046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZP80
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM182 may function in upon brown preadipocyte to adipocyte conversion. TMEM182 has some isoforms with MW 12-15 kDa and 26 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	FASEB Bioadv; TMEM182 inhibits myocardial differentiation of human iPS cells by maintaining the activated state of Wnt/β-catenin signaling through an increase in ILK expression; Authors - Hirofumi Morihara