TMEM231 Polyclonal antibody

TMEM231 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23731-1-AP

Synonyms

ALYE870, FLJ22167, hypothetical protein FLJ22167, PRO1886, TMEM231, Transmembrane protein 231

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Validation Data Gallery

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  IHC staining of human pancreas using 23731-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23731-1-AP (TMEM231 Antibody) at dilution of 1:50. (under 10x lens).

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  IHC staining of human pancreas using 23731-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23731-1-AP (TMEM231 Antibody) at dilution of 1:50. (under 40x lens).

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  IF Staining of MDCK using 23731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using TMEM231 antibody (23731-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23731-1-AP targets TMEM231 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM231 fusion protein Ag20669
• Full Name: hypothetical protein FLJ22167
• Calculated Molecular Weight: 345 aa, 39 kDa
• GenBank Accession Number: BC010609
• Gene Symbol: TMEM231
• Gene ID (NCBI): 79583
• RRID: AB_2879312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 231 (TMEM231) encodes a 36 kDa two-pass transmembrane protein that is part of the B9 complex and localizes to the base of the ciliary axoneme at the TZ. TMEM231 was first identified as a causative gene for MKS and participates in the formation of the diffusion barrier between the cilia and plasma membrane (PMID: 34912761, 37736303). Tmem231 mutant mice have developmental defects reminiscent of MKS patients, including corticomedullary kidney cysts, polydactyly and hepatic ductal plate malformation (PMID: 25869670).