TMEM24 Polyclonal antibody

TMEM24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25507-1-AP

Synonyms

C2CD2 like, C2CD2L, DLNB23, KIAA0285, TMEM24, Transmembrane protein 24

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Validation Data Gallery

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  WB analysis of HeLa using 25507-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25507-1-AP (TMEM24 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 25507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25507-1-AP (TMEM24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 25507-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25507-1-AP (TMEM24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 25507-1-AP is TMEM24 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM24 fusion protein Ag21913
• Full Name: C2CD2-like
• Calculated Molecular Weight: 706 aa, 76 kDa
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: BC022219
• Gene Symbol: C2CD2L
• Gene ID (NCBI): 9854
• RRID: AB_2880108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM24, also named as KIAA0285 and C2CD2L, is identified as a pancreatic islet-enriched protein that plays a key role in regulating glucose-sensitive INS release from the reserve pool of granules. This antibody detects TMEM24 protein with MW 70-100 kDa.

Publications

Species	Application	Title
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss