Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24361-1-AP targets TMEM25 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19964 Product name: Recombinant human TMEM25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-323 aa of BC051841 Sequence: LEPQIDGQTWAERALRENERHAFTCRVAGGPGTPRLAWYLDGQLQEASTSRLLSVGGEAFSGGTSTFTVTAHRAQHELNCSLQDPRSGRSANASVILNVQFKPEIAQVGAKYQEAQGPGLLVVLFALVRANPPANVTWIDQDGPVTVNTSDFLVLDAQNYPWLTNHTVQLQLRSLAHNLSVVATNDVGVTSASLPAPGPSRHPSLISSDSNNLKLNNVRLPRENMSLPSNLQLNDLTPDSRAVKPADRQMAQNNSRPELLDPEPGGLLTSRGFIRLPVLGYIYRVSSVSSDEIWL Predict reactive species |
| Full Name | transmembrane protein 25 |
| Calculated Molecular Weight | 366 aa, 39 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC051841 |
| Gene Symbol | TMEM25 |
| Gene ID (NCBI) | 84866 |
| RRID | AB_2879507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86YD3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM25 is a novel member of the immunoglobulin superfamily. BC042896 and AY358919 cDNAs are derived from human TMEM25 gene. TMEM25 isoform 1 (BC042896), consisting of exons 1-9, encodes a 366-aa transmembrane protein. TMEM25 isoform 2 (AY358919), consisting of exons 1-4 and 6-9, encodes a 322-aa secreted protein (PMID:15254712). Human TMEM25 gene are found to encode transmembrane-type as well as secreted-type proteins due to alternative splicing of exon-skipping type. TMEM25 mRNA is expressed in brain, including cerebellar cortex and hippocampus, as well as in neuroblastoma, brain tumors, and gastric cancer. TMEM25 is a target of pharmacogenomics in the field of oncology and regenerative medicine. TMEM25 mRNAs may be useful members of a panel of favorable prognostic and predictive markers for breast cancer( PMID:19776672).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM25 antibody 24361-1-AP | Download protocol |
| WB protocol for TMEM25 antibody 24361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TMEM25 Polyclonal antibody (Cat# 24361-1-AP) has 1 citation. It appears in the Journal of Clinical Investigation (IF 14.3), in a study demonstrating that TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. The research field is neuroscience, specifically neuronal signaling and NMDA receptor biology. The antibody was validated in human and mouse samples using WB, IF, and CoIP applications.
| Species | Application | Title |
|---|---|---|
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.
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