CoraLite®594-conjugated TMF1 Monoclonal antibody

TMF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G12F2

Cat no : CL594-67505

Synonyms

ARA160, TATA element modulatory factor, TMF, TMF1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67505

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 TMF1 antibody (CL594-67505, Clone: 3G12F2 ) at dilution of 1:200, CL488-Phalloidin (green).

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67505 targets TMF1 in IF applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMF1 fusion protein Ag17666
• Full Name: TATA element modulatory factor 1
• Calculated Molecular Weight: 1093 aa, 123 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC126123
• Gene Symbol: TMF1
• Gene ID (NCBI): 7110
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.