Filter: AllWBIPIHCIF/ICC

WB analysis using 14651-1-AP (same clone as 14651-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 14651-1-AP (same clone as 14651-1-PBS)
WB result of TMPO/LAP2 antibody (14651-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMPO/LAP2 transfected HeLa cells. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 14651-1-AP (same clone as 14651-1-PBS)
IP result of anti-TMPO/LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 14651-1-AP (same clone as 14651-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 14651-1-AP (same clone as 14651-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.
IF Staining of HeLa cells using 14651-1-AP (same clone as 14651-1-PBS)
IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

TMPO/LAP2 Polyclonal antibody, PBS Only

TMPO/LAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14651-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14651-1-PBS targets TMPO/LAP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6247 Product name: Recombinant human TMPO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC053675 Sequence: MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISAS Predict reactive species
Full Name	thymopoietin
Calculated Molecular Weight	51 kDa
Observed Molecular Weight	75 kDa, 51 kDa and 39 kDa
GenBank Accession Number	BC053675
Gene Symbol	TMPO/LAP2
Gene ID (NCBI)	7112
RRID	AB_2205440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42167
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).