TNFAIP1 Monoclonal antibody

TNFAIP1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60327-1-Ig

Clone No. 4F11B5

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

4F11B5, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2, BTB/POZ domain-containing protein TNFAIP1, EDP1, Tumor necrosis factor, alpha-induced protein 1, endothelial

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of HUVEC using 60327-1-Ig
HUVEC, HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 60327-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 60327-1-Ig (TNFAIP1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 60327-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung cancer using 60327-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 60327-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 60327-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60327-1-Ig (TNFAIP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HUVEC cells, HeLa cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60327-1-Ig targets TNFAIP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7873 Product name: Recombinant human TNFAIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 71-239 aa of BC001949 Sequence: ILIDRCGKHFGTILNYLRDDTITLPQNRQEIKELMAEAKYYLIQGLVNMCQSALQDKKDSYQPVCNIPIITSLKEEERLIESSTKPVVKLLYNRSNNKYSYTSNSDDHLLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKLAEVCCTSIVYATEKKQTKV Predict reactive species
Full Name	tumor necrosis factor, alpha-induced protein 1 (endothelial)
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC001949
Gene Symbol	TNFAIP1
Gene ID (NCBI)	7126
RRID	AB_2881437
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q13829
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TNFAIP1 antibody 60327-1-Ig	Download protocol
WB protocol for TNFAIP1 antibody 60327-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TNFAIP1 Monoclonal antibody (4F11B5), catalog number 60327-1-Ig, has 1 total citation. The cited publication appeared in the journal Oncotarget (2017, Impact Factor 5.31). The study investigated Mcl-1 stabilization conferring resistance to taxol in human gastric cancer, placing this citation within the research fields of oncology, gastric cancer biology, and chemoresistance mechanisms. The antibody was used in Western Blot (WB) with human samples.