TNFAIP8 Monoclonal antibody

TNFAIP8 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D2F7

Cat no : 67737-1-Ig

Synonyms

GG2 1, MDC 3.13, NDED, SCC S2, SCCS2, TNF alpha induced protein 8, TNF induced protein GG2 1, TNFAIP8

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Validation Data Gallery

• 

  WB analysis using 67737-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67737-1-Ig (TNFAIP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67737-1-Ig targets TNFAIP8 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TNFAIP8 fusion protein Ag8805
• Full Name: tumor necrosis factor, alpha-induced protein 8
• Calculated Molecular Weight: 198 aa, 23 kDa
• Observed Molecular Weight: 19-21 kDa
• GenBank Accession Number: BC007014
• Gene Symbol: TNFAIP8
• Gene ID (NCBI): 25816
• RRID: AB_2918506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFAIP8 (tumor necrosis factor, alpha-induced protein 8), also called SCC-S2/GG2-1/NDED, is associated with enhanced cell survival and inhibition of apoptosis. The induction of TNFAIP8 by TNF depends on the activation of NFκB. TNFAIP8 suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation. TNFAIP8 is expressed in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney at high levels, also expressed in various tumor tissues and all cancer cell lines tested.