TNFRSF6B Recombinant monoclonal antibody

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• TNFRSF6B Recombinant monoclonal antibody (87840-1-RR)

TNFRSF6B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87840-1-RR

Clone No.253457A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DcR3, Decoy receptor 3, Decoy receptor for Fas ligand, M68, TR6

• 

  WB analysis using 87840-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of ACHN using 87840-1-RR

  ACHN cells were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87840-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87840-1-RR against Mouse TNFRSF6B were performed. The affinity constant is 0.131 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: ACHN cells, SW480 cells, HCT 116 cells, SW 1990 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87840-1-RR targets TNFRSF6B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3110

  Product name: Recombinant human TNFRSF6B protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 30-300 aa of NM_003823.3

  Sequence: VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH

• Full Name: tumor necrosis factor receptor superfamily, member 6b, decoy
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_003823.3
• Gene Symbol: TNFRSF6B
• Gene ID (NCBI): 8771
• RRID: AB_3745781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95407
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tumor necrosis factor receptor super-family member 6b (TNFRSF6B) is over-expressed in various human cancers. TNFRSF6B mRNA and protein expression in the tumor tissues negatively correlated with apoptosis. Cell proliferation was decreased, cell cycle was arrested in G1/S-phase, apoptosis was increased, and migration ability was inhibited by anti-TNFRSF6B McAb in vitro. Anti-TNFRSF6B McAb could be useful to suppress proliferation and induce apoptosis in HCC. Thus, TNFRSF6B might be a critical, targeted therapy strategy for HCC (PMID: 20591579).