CoraLite® Plus 647 Anti-Mouse TNFRSF9/CD137 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL647-98182

  1x10^6 untreated or Con A treated mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD25/IL-2RA and 0.25 ug CoraLite® Plus 647 Anti-Mouse TNFRSF9/CD137 Rabbit RecAb (CL647-98182, Clone: 241647E11). Cells were not fixed.

TNFRSF9/CD137 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98182

Clone No.241647E11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241647E11, 4-1BB, 4-1BB ligand receptor, CD137, Ila

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Tested Applications

• Positive FC detected in: Con A treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98182 targets TNFRSF9/CD137 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: tumor necrosis factor receptor superfamily, member 9
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: NM_001077509.1
• Gene Symbol: Tnfrsf9
• Gene ID (NCBI): 21942
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20334
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)