Filter: AllWBIF/ICCAffinity

WB analysis using 87367-3-RR (same clone as 87367-3-PBS)
Transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 87367-3-RR (TNFSF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 87367-3-PBS in a different storage buffer formulation.
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Transfected HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 87367-3-RR (TNFSF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 87367-3-PBS in a different storage buffer formulation.
IF Staining of Transfected HEK-293 using 87367-3-RR (same clone as 87367-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using TNFSF15 antibody (87367-3-RR, Clone: 250983F1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 87367-3-PBS in a different storage buffer formulation.
Affinity of 87367-3-RR
Biolayer interferometry (BLl) kinetic assays of 87367-3-RR against Mouse TNFSF15 were performed. The affinity constant is below 1 pM.

Mouse TNFSF15 Recombinant monoclonal antibody, PBS Only

Name: Mouse TNFSF15 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 87367-3-PBS

TNFSF15 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87367-3-PBS

Clone No.250983F1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Tl1, Tl1, Vegi, TNF15, TNFSF 15, Vegi

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

87367-3-PBS targets TNFSF15 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg5273 Product name: Recombinant Mouse TNFSF15 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 61-252 aa of / Sequence: AGQLRVPGKDCMLRAITEERSEPSPQQVYSPPRGKPRAHLTIKKQTPAPHLKNQLSALHWEHDLGMAFTKNGMKYINKSLVIPESGDYFIYSQITFRGTTSVCGDISRGRRPNKPDSITVVITKVADSYPEPARLLTGSKSVCEISNNWFQSLYLGAMFSLEEGDRLMVNVSDISLVDYTKEDKTFFGAFLL Predict reactive species
Full Name	tumor necrosis factor (ligand) superfamily, member 15
Calculated Molecular Weight	28 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	/
Gene Symbol	Tnfsf15
Gene ID (NCBI)	326623
RRID	AB_3745488
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q5UBV8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

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