Tested Applications
| Positive WB detected in | SKOV-3 cells, SW 1990 cells, LoVo cells, Caki-1 cells, Caki-2 cells, SCaBER cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66450-1-Ig targets 4-1BBL/TNFSF9 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12768 Product name: Recombinant human TNFSF9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 53-254 aa of BC104805 Sequence: WAVSGARASPGSAASPRLREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPRSE Predict reactive species |
| Full Name | tumor necrosis factor (ligand) superfamily, member 9 |
| Calculated Molecular Weight | 254 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC104805 |
| Gene Symbol | TNFSF9 |
| Gene ID (NCBI) | 8744 |
| RRID | AB_2881819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P41273 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNFSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for 4-1BBL/TNFSF9 antibody 66450-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
4-1BBL/TNFSF9 monoclonal antibody (Cat# 66450-1-Ig) has 8 total citations. Journals include Science, Hepatology Communications, Aging, International Immunopharmacology, European Journal of Medical Research, BMC Cancer, Asia Pacific Journal of Clinical Oncology, and bioRxiv. Research fields span lysosomal degradation of membrane proteins, intrahepatic cholangiocarcinoma, pancreatic cancer metastasis, stomach adenocarcinoma, renal cell carcinoma, uveal melanoma, and non-small cell lung cancer.
| Species | Application | Title |
|---|---|---|
Hepatol Commun HBV infection effects prognosis and activates the immune response in intrahepatic cholangiocarcinoma | ||
Aging (Albany NY) TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.
| ||
Int Immunopharmacol TNFSF9 promotes metastasis of pancreatic cancer by regulating M2 polarization of macrophages through Src/FAK/p-Akt/IL-1β signaling
| ||
Eur J Med Res Integrative analysis of bulk and single-cell sequencing reveals TNFSF9 as a potential regulator in microsatellite instability stomach adenocarcinoma | ||
BMC Cancer Clinical significance of nonerythrocytic spectrin Beta 1 (SPTBN1) in human kidney renal clear cell carcinoma and uveal melanoma: a study based on Pan-Cancer Analysis |
