Cardiac Troponin I Monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP51198-1

  Sandwich ELISA standard curve of MP51198-1, Human Cardiac Troponin I Monoclonal Matched Antibody Pair - PBS only. 66376-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16758. 66376-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP51198-1

  The mean Cardiac Troponin I concentration was determined to be 220.78 ng/mL in mouse heart tissue extract based on a 7.10 mg/mL extract load.

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  Sample test of MP51198-1

  Cytometric bead array in cell lysate using MP51198-1, Cardiac Troponin I Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66376-2-PBS. Detection antibody: 66376-1-PBS. Cell lysate: Rabbit muscle, Rabbit heart.

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  Cytometric bead array standard curve of MP51198-1

  Cytometric bead array standard curve of MP51198-1, Cardiac Troponin I Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66376-2-PBS. Detection antibody: 66376-1-PBS. Standard:Ag16758. Range: 12.5-400 ng/mL.

Cardiac Troponin I Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 66376-2-PBS

Clone No.1C5E8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

cTnI, TNNI3

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Product Information

66376-2-PBS targets Cardiac Troponin I as part of a matched antibody pair:

MP51198-1: 66376-2-PBS capture and 66376-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16758

  Product name: Recombinant human TNNI3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-110 aa of BC096165

  Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE

• Full Name: troponin I type 3 (cardiac)
• Calculated Molecular Weight: 210 aa, 24 kDa
• GenBank Accession Number: BC096165
• Gene Symbol: Cardiac Troponin I
• Gene ID (NCBI): 7137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19429
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.