Tested Applications
| Positive WB detected in | pig heart tissue, human heart tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15513-1-AP targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK Predict reactive species |
| Full Name | troponin T type 2 (cardiac) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC002653 |
| Gene Symbol | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_2206563 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. cTnT is commonly used as a marker of cardiomyogenic differentiation; in H9C2 rat cardiomyoblasts, its expression typically increases upon differentiation induction. Similar to H9C2, immortalized cardiomyocyte cell lines such as AC16 and HL-1 also show changes in the expression of cardiac markers (e.g., cTnT/TNNT2) upon differentiation induction-cardiac markers increase significantly in differentiated AC16 and H9C2 cells, whereas HL-1 already possesses a certain degree of sarcomeric structure-yet none of the three can fully recapitulate the transcriptomic and functional phenotypes of mature cardiomyocytes. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cardiac Troponin T antibody 15513-1-AP | Download protocol |
| IHC protocol for Cardiac Troponin T antibody 15513-1-AP | Download protocol |
| WB protocol for Cardiac Troponin T antibody 15513-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cardiac Troponin T Polyclonal antibody (Cat# 15513-1-AP) has 116 citations. Publications appear in high-impact journals including Nature, Nat Commun, Cell Stem Cell, Sci Transl Med, J Clin Invest, Biomaterials, Adv Sci, Mol Ther, EMBO J, and J Mol Cell Cardiol, among others. Research fields span cardiac development, myocardial infarction/ischemia-reperfusion injury, cardiac hypertrophy, cardiomyocyte differentiation from stem cells, cardiac regeneration, heart failure, and cardiac fibrosis, with applications primarily in IF, WB, and IHC across human, mouse, rat, and other species.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Poly(I:C) preconditioning protects the heart against myocardial ischemia/reperfusion injury through TLR3/PI3K/Akt-dependent pathway. | ||
Eur Heart J Left bundle branch vs biventricular pacing: mechanistic insights from a canine model. | ||
Cell Stem Cell Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation | ||
Nat Commun N-Cadherin promotes cardiac regeneration by potentiating pro-mitotic β-Catenin signaling in cardiomyocytes | ||
Nat Commun Single-nuclei multiomics analysis identifies abnormal cardiomyocytes in a murine model of cardiac development |
Reviews
What customers say
Two reviewers rated this antibody positively. One used it for Western Blot at 1:1000 dilution on iPS-CM cells, loading 20 µg of protein with a 2-hour transfer at 4 °C/90 V and overnight antibody incubation at 4 °C, and gave it 5 stars. The other applied it for immunohistochemistry at 1:50 dilution on mouse embryonic heart tissue, noting it "works well," and gave it 4 stars. Overall, users report reliable performance in both WB and IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rebecca (Verified Customer) (03-16-2022) | 20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.
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Lianjie (Verified Customer) (07-26-2019) | This antibody works well.
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