Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15513-1-AP

Synonyms

Cardiac Troponin T, CMH2, cTnT, TNNT2, TnTC



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15513-1-AP targets Cardiac Troponin T in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag7716
Full Name troponin T type 2 (cardiac)
Calculated molecular weight 36 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC002653
Gene symbol TNNT2
Gene ID (NCBI) 7139
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
IHC protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
IF protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Brahma safeguards canalization of cardiac mesoderm differentiation.

Authors - Swetansu K Hota
mouseIHC

Dev Cell

A Multifunctional Protein, EWS, Is Essential for Early Brown Fat Lineage Determination.

Authors - Park Jun Hong JH
mouseIF

Nat Chem Biol

Cell-type-specific labeling and profiling of glycans in living mice.

Authors - Xinqi Fan
ratWB

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
human,mouseIF

Biomaterials

Direct in vivo reprogramming with non-viral sequential targeting nanoparticles promotes cardiac regeneration.

Authors - Qiaozi Wang
humanWB

Theranostics

Engineering extracellular vesicles with platelet membranes fusion enhanced targeted therapeutic angiogenesis in a mouse model of myocardial ischemia reperfusion.

Authors - Qiyu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rebecca (Verified Customer) (03-16-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: iPS-CM
Cardiac Troponin T Antibody Western Blot validation (1:1000 dilution) in iPS-CM (Cat no:15513-1-AP)
FH

Lianjie (Verified Customer) (07-26-2019)

This antibody works well.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse embryonic heart
Cardiac Troponin T Antibody Immunohistochemistry, validation (1:50 dilution) in Mouse embryonic heart (Cat no:15513-1-AP)