Cardiac Troponin T Polyclonal antibody

Cardiac Troponin T Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15513-1-AP

Synonyms

Cardiac Troponin T, CMH2, cTnT, TNNT2, TnTC



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15513-1-AP targets Cardiac Troponin T in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cardiac Troponin T fusion protein Ag7716
Full Name troponin T type 2 (cardiac)
Calculated molecular weight 36 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC002653
Gene symbol TNNT2
Gene ID (NCBI) 7139
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
IHC protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
IF protocol for Cardiac Troponin T antibody 15513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Clin Sci (Lond)

Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling.

Authors - Yifang Xie
humanIF

EBioMedicine

Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.

Authors - Chunyan Li
humanIF

ACS Nano

Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Authors - Yiqi Gong
ratFC,IF

Stem Cells Int

5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells.

Authors - XiaoLin Sun
mouseIF

Signal Transduct Target Ther

Poly(I:C) preconditioning protects the heart against myocardial ischemia/reperfusion injury through TLR3/PI3K/Akt-dependent pathway.

Authors - Erya Chen
mouseIF

Adv Sci (Weinh)

Platelet-Like Fusogenic Liposome-Mediated Targeting Delivery of miR-21 Improves Myocardial Remodeling by Reprogramming Macrophages Post Myocardial Ischemia-Reperfusion Injury.

Authors - Haipeng Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lianjie (Verified Customer) (07-26-2019)

This antibody works well.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse embryonic heart
Cardiac Troponin T Antibody Immunohistochemistry, validation (1:50 dilution) in Mouse embryonic heart (Cat no:15513-1-AP)