TNNT3 Polyclonal antibody, PBS Only

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• TNNT3 Polyclonal antibody, PBS Only (19729-1-PBS)

• 

  WB analysis of rat skeletal muscle using 19729-1-AP (same clone as 19729-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19729-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 19729-1-AP (same clone as 19729-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19729-1-AP (TNNT3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19729-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 19729-1-AP (same clone as 19729-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19729-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 19729-1-AP (same clone as 19729-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19729-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 19729-1-AP (same clone as 19729-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19729-1-AP (TNNT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19729-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 19729-1-AP (same clone as 19729-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19729-1-AP (TNNT3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19729-1-PBS in a different storage buffer formulation.

TNNT3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19729-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Beta-TnTF, Fast skeletal muscle troponin T, Troponin T3 skeletal fast, Troponin T-FS

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Product Information

19729-1-PBS targets TNNT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: troponin T type 3 (skeletal, fast)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_001042780
• Gene Symbol: TNNT3
• Gene ID (NCBI): 7140
• RRID: AB_2878601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P45378
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The TNNT3 gene encodes the fast skeletal muscle isoform of troponin T, a key protein subunit of the troponin complex responsible for regulating skeletal muscle contraction in a calcium-dependent manner. This gene produces a protein that anchors the troponin complex to tropomyosin on the thin filaments of sarcomeres, playing a crucial role in the conformational changes that permit actin-myosin cross-bridge formation during muscle contraction. Tissue-specific expression is largely restricted to fast-twitch skeletal muscles, where alternative splicing generates multiple developmentally regulated isoforms. Clinically, dominant pathogenic mutations in TNNT3 are associated with distal arthrogryposis multiplex congenita type 2B (DA2B), also known as Sheldon-Hall syndrome, characterized by congenital joint contractures, while rare recessive variants have been linked to congenital myopathies featuring nemaline rods and fiber atrophy.