TOB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TOB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14915-1-AP

Synonyms

Transducer of erbB-2 1, Transducer of erbB 2 1, TOB, Protein Tob1, PIG49

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Validation Data Gallery

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  WB analysis of HeLa using 14915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14915-1-AP (TOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14915-1-AP

  IP result of anti-TOB1 (IP:14915-1-AP, 4ug; Detection:14915-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14915-1-AP targets TOB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOB1 fusion protein Ag6714
• Full Name: transducer of ERBB2, 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC031406
• Gene Symbol: TOB1
• Gene ID (NCBI): 10140
• RRID: AB_10896496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

Publications

Species	Application	Title
human	WB	Oncotarget; Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.; Authors - Jin Zhu
mouse	IHC	RNA Biol; NEDD4 triggers FOXA1 ubiquitination and promotes colon cancer progression under microRNA-340-5p suppression and ATF1 upregulation.; Authors - Meng Yue
human	WB	RNA Biol; Preferential translation of p53 target genes.; Authors - Miharu Hisaoka
human	WB	Am J Transl Res; Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.; Authors - Dong Wang
human	WB,IHC	Cancer Med; TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.; Authors - Yuru Bai
mouse,human	WB,IF,IP	Commun Biol; TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression; Authors - Zhongxia Yu ; KO Validated