Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, mouse thymus tissue, HeLa cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16203-1-AP targets TOE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9172 Product name: Recombinant human TOE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-510 aa of BC009364 Sequence: NDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS Predict reactive species |
| Full Name | target of EGR1, member 1 (nuclear) |
| Calculated Molecular Weight | 510 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC009364 |
| Gene Symbol | TOE1 |
| Gene ID (NCBI) | 114034 |
| RRID | AB_2207278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96GM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOE1 antibody 16203-1-AP | Download protocol |
| WB protocol for TOE1 antibody 16203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is anti-TOE1 (catalog #16203-1-AP), with 4 total citations. These appear in Scientific Reports, Journal of Biological Chemistry, Theriogenology, and Biochimica et Biophysica Acta (General Subjects). The associated research fields span hepatocellular carcinoma, profilin1-associated ALS, mouse early embryonic development (morula-to-blastocyst transition), and gastric cancer cell cycle regulation. Applications include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Sci Rep Transcriptomic analysis reveals the potential role of TOE1 in hepatocellular carcinoma.
| ||
J Biol Chem Abnormal regulation of membrane-less organelles contributes to profilin1-associated ALS | ||
Theriogenology Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice | ||
Biochim Biophys Acta Gen Subj TOE1 deadenylase inhibits gastric cancer cell proliferation by regulating cell cycle progression
|
Reviews
What customers say
The single review rates this antibody 5 stars. The user reports it works superbly for detecting endogenous human TOE1 protein by Western blotting in K562 cells (chronic myeloid leukaemia) at a 1/1000 dilution. The blot demonstrated clear detection across wild-type, empty-vector control, and overexpression lanes, as well as effective knockdown using multiple TOE1 shRNA constructs, confirming strong specificity and reliable performance for both overexpression and loss-of-function studies.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rhys (Verified Customer) (08-26-2019) | Superb antibody that works well for the detection for endogenous human TOE1 protein by western blotting.Lane 1: K562 wtGapLane 2: K562 control (empty vector)Lane 3: K562 TOE1 overexpressionGapLane 4: K562 scrambled shRNA controlLane 5: K562 TOE1 shRNA #1Lane 6: K562 TOE1 shRNA #2Lane 7: K562 TOE1 shRNA #3Lane 8: K562 TOE1 shRNA #4Lane 9: K562 TOE1 shRNA #5
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