TOE1 Polyclonal antibody

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  WB analysis using 16203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16203-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16203-1-AP (TOE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16203-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOE1 antibody (16203-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TOE1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16203-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Target of EGR1 protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse thymus tissue, HeLa cells, K-562 cells, Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16203-1-AP targets TOE1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9172

  Product name: Recombinant human TOE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 162-510 aa of BC009364

  Sequence: NDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS

• Full Name: target of EGR1, member 1 (nuclear)
• Calculated Molecular Weight: 510 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC009364
• Gene Symbol: TOE1
• Gene ID (NCBI): 114034
• RRID: AB_2207278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96GM8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Theriogenology; Knockdown of Toe1 causes developmental arrest during the morula-to-blastocyst transition in mice; Authors - Hongcheng Wang
human	WB	Biochim Biophys Acta Gen Subj; TOE1 deadenylase inhibits gastric cancer cell proliferation by regulating cell cycle progression; Authors - Xiao-Lin Sun ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rhys (Verified Customer) (08-26-2019)

Superb antibody that works well for the detection for endogenous human TOE1 protein by western blotting.Lane 1: K562 wtGapLane 2: K562 control (empty vector)Lane 3: K562 TOE1 overexpressionGapLane 4: K562 scrambled shRNA controlLane 5: K562 TOE1 shRNA #1Lane 6: K562 TOE1 shRNA #2Lane 7: K562 TOE1 shRNA #3Lane 8: K562 TOE1 shRNA #4Lane 9: K562 TOE1 shRNA #5

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: K562 cells (Chronic Myeloid Leukaemia)

TOE1 Antibody Western Blot, validation (1/1000 dilution) in K562 cells (Chronic Myeloid Leukaemia) (Cat no:16203-1-AP)