CoraLite®594-conjugated TOM20 Recombinant antibody

• 

  IF Staining of HepG2 using CL594-80501

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 TOM20 antibody (CL594-80501, Clone: 1D22 ) at dilution of 1:200, CL488-Phalloidin (green).

TOM20 Recombinant Antibody for IF/ICC

Cat No. CL594-80501

Clone No.1D22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken, zebrafish

Applications

IF/ICC

Synonyms

TOMM20, 1D22, KIAA0016, MAS20, MOM19

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80501 targets TOM20 in IF/ICC applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

• Tested Reactivity: human, mouse, rat, pig, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOM20 fusion protein Ag2378
• Full Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Calculated Molecular Weight: 145 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC000882
• Gene Symbol: TOM20
• Gene ID (NCBI): 9804
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15388
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.