Tom22 Polyclonal antibody
Tom22 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 11278-1-AP
|Positive WB detected in||HeLa, K-562 cells, HeLa cells, mouse liver tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11278-1-AP targets Tom22 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Tom22 fusion protein Ag1797|
|Full Name||translocase of outer mitochondrial membrane 22 homolog (yeast)|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15-22 kDa|
|GenBank accession number||BC009363|
|Gene ID (NCBI)||56993|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises the 22 kDa protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).
|Product Specific Protocols|
|WB protocol for Tom22 antibody 11278-1-AP||Download protocol|
|IHC protocol for Tom22 antibody 11278-1-AP||Download protocol|
|IF protocol for Tom22 antibody 11278-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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