Tom22 Polyclonal antibody

Tom22 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11278-1-AP

Synonyms

1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22



Tested Applications

Positive WB detected inHeLa, K-562 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11278-1-AP targets Tom22 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tom22 fusion protein Ag1797
Full Name translocase of outer mitochondrial membrane 22 homolog (yeast)
Calculated molecular weight 15 kDa
Observed molecular weight 15-22 kDa
GenBank accession numberBC009363
Gene symbol TOMM22
Gene ID (NCBI) 56993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Protocols

Product Specific Protocols
WB protocol for Tom22 antibody 11278-1-APDownload protocol
IHC protocol for Tom22 antibody 11278-1-APDownload protocol
IF protocol for Tom22 antibody 11278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hum Genet

Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.

Authors - Xiujuan Wei
humanWB

Mol Biol Cell

Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

Authors - Shan Xu
humanWB

J Genet Genomics

HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration.

Authors - Yu Sun
humanWB,IF

Cell Discov

Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.

Authors - Fan Yu
humanWB

Cell Res

Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.

Authors - Bo Zhou