Tom22 Polyclonal antibody

Tom22 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11278-1-AP

Synonyms

1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22



Tested Applications

Positive WB detected inHeLa, K-562 cells, human liver tissue, human lung tissue, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

11278-1-AP targets Tom22 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tom22 fusion protein Ag1797
Full Name translocase of outer mitochondrial membrane 22 homolog (yeast)
Calculated molecular weight 15 kDa
Observed molecular weight 15-22 kDa
GenBank accession numberBC009363
Gene symbol TOMM22
Gene ID (NCBI) 56993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Tom22 antibody 11278-1-APDownload protocol
IHC protocol for Tom22 antibody 11278-1-APDownload protocol
IF protocol for Tom22 antibody 11278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hum Genet

Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.

Authors - Xiujuan Wei
humanWB

Mol Biol Cell

Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.

Authors - Shan Xu
humanWB

J Genet Genomics

HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration.

Authors - Yu Sun
humanWB

Cell Res

Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.

Authors - Bo Zhou