TOP1 Polyclonal antibody

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  WB analysis using 20705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20705-1-AP (TOP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 20705-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20705-1-AP (TOP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOP1 antibody (20705-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

TOP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20705-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Top-I, TOPI, EC:5.6.2.1, DNA topoisomerase I, DNA topoisomerase 1

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

20705-1-AP targets TOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: topoisomerase (DNA) I
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: NM_003286
• Gene Symbol: TOP1
• Gene ID (NCBI): 7150
• RRID: AB_10695762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11387
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nat Commun; Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.; Authors - Bo Zhao
human	WB, IF	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
human	WB	J Transl Med; Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1; Authors - Ruijiao Chen
human	WB	Oncotarget; Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.; Authors - Xia Lingzi
human	WB	J Cell Mol Med; EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.; Authors - Wenbing Wu
mouse	WB	iScience; Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.; Authors - Xue Hao