TOP1 Polyclonal antibody

TOP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 20705-1-AP


DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, Top-I, TOPO1, topoisomerase (DNA) I

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20705-1-AP targets TOP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name topoisomerase (DNA) I
Calculated molecular weight 91 kDa
Observed molecular weight 90 kDa
GenBank accession numberNM_003286
Gene symbol TOP1
Gene ID (NCBI) 7150
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.


Product Specific Protocols
WB protocol for TOP1 antibody 20705-1-APDownload protocol
IHC protocol for TOP1 antibody 20705-1-APDownload protocol
IF protocol for TOP1 antibody 20705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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