TOP1 Polyclonal antibody

TOP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 20705-1-AP


DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, Top-I, TOPO1, topoisomerase (DNA) I

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20705-1-AP targets TOP1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name topoisomerase (DNA) I
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberNM_003286
Gene Symbol TOP1
Gene ID (NCBI) 7150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.


Product Specific Protocols
WB protocol for TOP1 antibody 20705-1-APDownload protocol
IHC protocol for TOP1 antibody 20705-1-APDownload protocol
IF protocol for TOP1 antibody 20705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.

Authors - Bo Zhao
humanWB, IF

Cell Chem Biol

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Authors - Amy Flor

J Transl Med

Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1

Authors - Ruijiao Chen


Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.

Authors - Xia Lingzi

J Cell Mol Med

EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.

Authors - Wenbing Wu


Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.

Authors - Xue Hao