|Positive WB detected in||L02 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20705-1-AP targets TOP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||topoisomerase (DNA) I|
|Calculated molecular weight||91 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||NM_003286|
|Gene ID (NCBI)||7150|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.
Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.
Cell Chem Biol
Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.
J Transl Med
Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1
Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.
J Cell Mol Med
EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.
Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.