TOP1 Polyclonal antibody

TOP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20705-1-AP

Synonyms

DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, Top-I, TOPO1, topoisomerase (DNA) I



Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20705-1-AP targets TOP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name topoisomerase (DNA) I
Calculated molecular weight 91 kDa
Observed molecular weight 90 kDa
GenBank accession numberNM_003286
Gene symbol TOP1
Gene ID (NCBI) 7150
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.

Protocols

Product Specific Protocols
WB protocol for TOP1 antibody 20705-1-APDownload protocol
IHC protocol for TOP1 antibody 20705-1-APDownload protocol
IF protocol for TOP1 antibody 20705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

IFN-γ restores the impaired function of RNase L and induces mitochondria-mediated apoptosis in lung cancer.

Authors - Huijing Yin
humanWB

Oncol Lett

PMID: 28781667

Authors - Ling Duan

Oncol Rep

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Authors - Lei Gao
humanWB

Chem Biol Interact

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Authors - Jingjun Wu
humanWB

Sci Rep

Transcription modulation by CDK9 regulates inflammatory genes and RIPK3-MLKL-mediated necroptosis in periodontitis progression.

Authors - Jiao Li
mouseWB

iScience

Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.

Authors - Xue Hao