TOP1MT Polyclonal antibody

• Featured Product
• KD/KO Validated

TOP1MT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16540-1-AP

Synonyms

2900052H09Rik, TOP1MT

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Validation Data Gallery

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  WB analysis of COLO 320 using 16540-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16540-1-AP (TOP1MT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

16540-1-AP targets TOP1MT in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOP1MT fusion protein Ag9857
• Full Name: topoisomerase (DNA) I, mitochondrial
• Calculated Molecular Weight: 601 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC052285
• Gene Symbol: TOP1MT
• Gene ID (NCBI): 116447
• RRID: AB_2878274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; The human mitochondrial genome contains a second light strand promoter; Authors - Benedict G Tan ; KD Validated
human	WB	Cancer Metab; Deficiency of TOP1MT enhances glycolysis through the stimulation of PDK4 expression in gastric cancer; Authors - Hongqiang Wang