TOP2A Polyclonal antibody

• Featured Product
• KD/KO Validated

TOP2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 24641-1-AP

Synonyms

TOPII, TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha

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Validation Data Gallery

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  WB analysis using 24641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24641-1-AP

  WB result of TOP2A antibody (24641-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.

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  WB analysis of HeLa using 24641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24641-1-AP

  IP result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human pancreas cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

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  IF Staining of A431 using 24641-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (24641-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, MDA-MB-231 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

24641-1-AP targets TOP2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOP2A fusion protein Ag19551
• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 1531 aa, 174 kDa
• Observed Molecular Weight: 174 kDa
• GenBank Accession Number: BC140791
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• RRID: AB_2879651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC.; Authors - Meng-Yu Chen
human	WB,IF,IP	Mol Biol Cell; SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.; Authors - Ryuta Watanabe
		Dev Biol; Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.; Authors - Jacinta H Martin
human	WB	Chem Biol Interact; SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.; Authors - Jingjun Wu
		Oncol Rep; Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study.; Authors - Jun-Song Liu
human	IHC	J Clin Neurosci; High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas.; Authors - Xingwang Zhou