Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24641-1-AP targets TOP2A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19551 Product name: Recombinant human TOP2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1187-1531 aa of BC140791 Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF Predict reactive species |
| Full Name | topoisomerase (DNA) II alpha 170kDa |
| Calculated Molecular Weight | 1531 aa, 174 kDa |
| Observed Molecular Weight | 174 kDa |
| GenBank Accession Number | BC140791 |
| Gene Symbol | TOP2A |
| Gene ID (NCBI) | 7153 |
| RRID | AB_2879651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11388 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOP2A antibody 24641-1-AP | Download protocol |
| IHC protocol for TOP2A antibody 24641-1-AP | Download protocol |
| IP protocol for TOP2A antibody 24641-1-AP | Download protocol |
| WB protocol for TOP2A antibody 24641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TOP2A Polyclonal antibody (Cat# 24641-1-AP) has 26 citations spanning journals including Cell, EMBO Reports, Cell Chemical Biology, Cell Death & Discovery, PLoS Genetics, iScience, and Molecular Biology of the Cell. Research fields cover cancer biology (breast, gastric, ovarian, prostate, colorectal, thyroid, and oral cancers), cell proliferation, DNA damage repair, inflammation, neurodegeneration, stem cell biology, toxicology, and developmental biology.
| Species | Application | Title |
|---|---|---|
Cell Comparative proteomic landscapes elucidate human preimplantation development and failure | ||
Cell Mol Biol Lett STUB1 downregulates TOP2A through a dual mechanism of ubiquitination and FOXM1-mediated transcription repression, suppressing breast cancer growth and enhancing sensitivity to chemotherapy. | ||
Cell Death Discov Kinase activity profiling identifies putative downstream targets of cGMP/PKG signaling in inherited retinal neurodegeneration. | ||
Cell Chem Biol Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation | ||
Stem Cell Res Ther A single-cell transcriptomic atlas of human lens epithelium: identification and functional insights into lens stem/progenitor cells | ||
J Ethnopharmacol Yang-Xue-An-Tai Decoction Treats URSA by regulating the differentiation of proliferating decidual stromal cells through multiple targets. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user reported consistent, excellent results over a year of use in PhD research, specifically for TOP2A protein detection in cervical cancer cells via ChIP. The other highlighted its effectiveness as a capture antibody in point-of-care test applications. Overall, users find this antibody reliable and high-performing across different experimental contexts.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sayeh (Verified Customer) (05-27-2025) | I have been using this antibody for a year in my PhD research now and have gotten amazing results. I stripe this polyclonal ab on NC membranes for TOP2A protein detection in cervical cells.
|
Sayeh (Verified Customer) (01-31-2025) | This antibody works perfectly as capture antibody for point-of-care tests.
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