TOP2A Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82738-4-RR (same clone as 82738-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82738-4-RR (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82738-4-RR (same clone as 82738-4-PBS)

  IP result of anti-TOP2A (IP:82738-4-RR, 4ug; Detection:82738-4-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82738-4-RR (same clone as 82738-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-4-RR (TOP2A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 82738-4-RR (same clone as 82738-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (82738-4-RR, Clone: 1J18 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82738-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82738-4-RR against Human TOP2A were performed. The affinity constant is 0.928 nM.

TOP2A Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 82738-4-PBS

Clone No.1J18

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

1J18, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

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Product Information

82738-4-PBS targets TOP2A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19551

  Product name: Recombinant human TOP2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1187-1531 aa of BC140791

  Sequence: QDEQVGLPGKGGKAKGKKTQMAEVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEKKQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF

• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 1531 aa, 174 kDa
• Observed Molecular Weight: ~170 kDa
• GenBank Accession Number: BC140791
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11388
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.