TOP2A Recombinant antibody

TOP2A Recombinant Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1J18

Cat no : 82738-4-RR

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

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Validation Data Gallery

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  WB analysis using 82738-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82738-4-RR (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82738-4-RR

  IP result of anti-TOP2A (IP:82738-4-RR, 4ug; Detection:82738-4-RR 1:2000) with HeLa cells lysate 1280 ug.

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  IF Staining of A431 using 82738-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (82738-4-RR, Clone: 1J18 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), (red).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HEK-293 cells, A431 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82738-4-RR targets TOP2A in WB, IP, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOP2A fusion protein Ag19551
• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 1531 aa, 174 kDa
• Observed Molecular Weight: ~170 kDa
• GenBank Accession Number: BC140791
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.