TOP2A-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

TOP2A-Specific Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 20233-1-AP

Synonyms

TOP2A, TOP2, TOP II, EC:5.6.2.2, DNA topoisomerase 2-alpha

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Validation Data Gallery

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  WB analysis of HepG2 using 20233-1-AP

  WB result of TOP2A-Specific antibody (20233-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A-Specific transfected HepG2 cells.

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  WB analysis using 20233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 20233-1-AP

  IP result of anti-TOP2A-Specific (IP:20233-1-AP, 4ug; Detection:20233-1-AP 1:8000) with L02 cells lysate 1040 ug.

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  IHC staining of human ovary cancer using 20233-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20233-1-AP (TOP2A-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

20233-1-AP targets TOP2A-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 174 kDa
• Observed Molecular Weight: 170-177 kDa
• GenBank Accession Number: NM_001067
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• RRID: AB_10664923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publications

Species	Application	Title
mouse	IF	Science; Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.; Authors - Anand Minajigi
mouse	WB	Nat Commun; Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability; Authors - Zaili Luo
mouse	IHC	Research (Wash D C); Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation; Authors - Chao Song
human	WB	J Cell Biol; LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.; Authors - Yilin Fan
human	WB	Oncotarget; TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.; Authors - Xuefeng Li ; KD Validated
human	WB	J Med Chem; Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.; Authors - Xiaohong Yang