Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20233-1-AP targets TOP2A-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | topoisomerase (DNA) II alpha 170kDa |
| Calculated Molecular Weight | 174 kDa |
| Observed Molecular Weight | 170-177 kDa |
| GenBank Accession Number | NM_001067 |
| Gene Symbol | TOP2A |
| Gene ID (NCBI) | 7153 |
| RRID | AB_10664923 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11388 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOP2A-Specific antibody 20233-1-AP | Download protocol |
| IHC protocol for TOP2A-Specific antibody 20233-1-AP | Download protocol |
| IP protocol for TOP2A-Specific antibody 20233-1-AP | Download protocol |
| WB protocol for TOP2A-Specific antibody 20233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TOP2A antibody, Cat# 20233-1-AP, has 34 total citations. Journals include Science, Nature Communications, Nucleic Acids Research, J Exp Clin Cancer Res, Int J Biol Sci, J Cell Biol, FASEB J, J Biol Chem, Oncotarget, and others. Research fields span chromosome biology, DNA replication, spindle assembly, cancer (hepatocellular, gastric, colorectal, prostate, renal, glioblastoma, leukemia), drug resistance, ferroptosis, liver disease, and heart failure.
| Species | Application | Title |
|---|---|---|
Science Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. | ||
Nat Commun Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability | ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. | ||
Nucleic Acids Res The SMC5/SMC6 complex is critical for resolving R-loop-induced transcription-replication conflicts. | ||
J Exp Clin Cancer Res 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase. |
