TOP2A-Specific Recombinant monoclonal antibody

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• TOP2A-Specific Recombinant monoclonal antibody (82738-10-RR)

TOP2A-Specific Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 82738-10-RR

Clone No.253104A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

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  SEC-HPLC of 82738-10-RR

  The purity of Human TOP2A-Specific Recombinant monoclonal antibody (Cat. No. 82738-10-RR) was greater than 95% as determined by SEC-HPLC.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human tonsillitis using 82738-10-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 82738-10-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82738-10-RR (TOP2A-Specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 82738-10-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TOP2A-Specific antibody (82738-10-RR, Clone: 253104A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82738-10-RR targets TOP2A-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 174 kDa
• GenBank Accession Number: NM_001067
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• RRID: AB_3743420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11388
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.