CoraLite® Plus 488-conjugated TOP2A-Specific Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated TOP2A-Specific Recombinant monoclonal antibody (CL488-82738-10)

TOP2A-Specific Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-82738-10

Clone No.253104A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

TOP2A, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

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  IF Staining of U2OS using CL488-82738-10

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 TOP2A-Specific antibody (CL488-82738-10, Clone: 253104A1 ) at dilution of 1:200, CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82738-10 targets TOP2A-Specific in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 174 kDa
• GenBank Accession Number: NM_001067
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11388
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.