TOP3A Polyclonal antibody

• Featured Product
• KD/KO Validated

TOP3A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 14525-1-AP

Synonyms

DNA topoisomerase 3 alpha, DNA topoisomerase III alpha, TOP3, TOP3A, topoisomerase (DNA) III alpha

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Validation Data Gallery

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  WB analysis of K-562 using 14525-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14525-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14525-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14525-1-AP

  IP result of anti-TOP3A (IP:14525-1-AP, 4ug; Detection:14525-1-AP 1:500) with K-562 cells lysate 3600ug.

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 25 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

14525-1-AP targets TOP3A in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOP3A fusion protein Ag6010
• Full Name: topoisomerase (DNA) III alpha
• Calculated Molecular Weight: 1001 aa, 112 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC051748
• Gene Symbol: TOP3A
• Gene ID (NCBI): 7156
• RRID: AB_2205881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.

Publications

Species	Application	Title
human	WB	Mol Cell; BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint DNA molecule resolution; Authors - Kaima Tsukada
human	WB	Mol Cell; TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.; Authors - Andrew N Blackford
human	WB	Mol Cell; Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.; Authors - Thomas J Nicholls ; KD Validated
		Sci Adv; The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.; Authors - Min Jiang
human	WB	Nat Commun; The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.; Authors - Ann-Marie K Shorrocks
human	WB	Nucleic Acids Res; PMID: 30169847; Authors - Anu Hangas

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (01-23-2023)

Strong and crisp band.

• Applications: Western Blot