Tested Applications
| Positive WB detected in | K-562 cells, HL-60 cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14525-1-AP targets TOP3A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, goat, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6010 Product name: Recombinant human TOP3A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 652-1001 aa of BC051748 Sequence: PEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR Predict reactive species |
| Full Name | topoisomerase (DNA) III alpha |
| Calculated Molecular Weight | 1001 aa, 112 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC051748 |
| Gene Symbol | TOP3A |
| Gene ID (NCBI) | 7156 |
| RRID | AB_2205881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13472 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.
Publications
What published studies show
Anti-TOP3A Rabbit Polyclonal Antibody (Cat# 14525-1-AP) has 36 citations across journals including Nature Cell Biology, Nature Communications, Molecular Cell, Nucleic Acids Research, Cell Reports, EMBO Journal, EMBO Molecular Medicine, American Journal of Human Genetics, Journal of Cell Biology, and Science Advances. Research fields encompass mitochondrial DNA replication, genome stability, DNA repair, telomere biology, Bloom syndrome, Fanconi anemia, and topoisomerase function.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mitochondrial DNA replication stress triggers a pro-inflammatory endosomal pathway of nucleoid disposal
| ||
Cancer Res HERC2 Facilitates BLM and WRN Helicase Complex Interaction with RPA to Suppress G-Quadruplex DNA. | ||
Nat Commun Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes | ||
Nat Commun The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks. | ||
Nat Commun Centromere protection requires strict mitotic inactivation of the Bloom syndrome helicase complex | ||
Reviews
What customers say
The product received a 5-star rating for Western Blot applications. The reviewer reported obtaining a strong and crisp band, indicating high specificity and sensitivity in their experiment. Overall, the feedback reflects satisfaction with the antibody's performance in detecting the target protein clearly and reliably.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Andrea (Verified Customer) (01-23-2023) | Strong and crisp band.
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