TOR2A Polyclonal antibody

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  WB analysis of mouse brain using 19511-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19511-1-AP (TOR2A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19511-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19511-1-AP (TOR2A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 19511-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19511-1-AP (TOR2A antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19511-1-AP

  Immunofluorescent analysis of HepG2 cells using 19511-1-AP (TOR2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

TOR2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19511-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TORP1, Salusin-beta, Salusin-alpha, PSEC0218, Prosalusin

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19511-1-AP targets TOR2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: torsin family 2, member A
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_001085347
• Gene Symbol: TOR2A
• Gene ID (NCBI): 27433
• RRID: AB_10642949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N2E6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOR2A, also named as TORP1, UNQ6408/PRO21181, HEMBA1005096 and PSEC0218, belongs to the clpA/clpB family and Torsin subfamily. TOR2A contain two peptides Salusin-alpha and Salusin-beta. Salusins -alpha and -beta may be endocrine and/or paracrine factors able to increase intracellular calcium concentrations and induce cell mitogenesis. Salusins may also be potent hypotensive peptides.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Dis; Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy.; Authors - Ming-Xia Zhao
human	WB	Cell Death Dis; REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.; Authors - Yunshang Chen