Multi-rAb® TORC2 Multi-Recombinant antibody

• Home
• Products
• Primary Antibodies
• Multi-rAb® TORC2 Multi-Recombinant antibody (RMX00047)

• 

  WB analysis using RMX00047

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00047 (TORC2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using RMX00047

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using RMX00047 (CRTC2,TORC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using RMX00047

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CRTC2,TORC2 antibody (RMX00047) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TORC2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00047

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CRTC2, CRTC2,TORC2, CREB-regulated transcription coactivator 2, TORC 2, Transducer of CREB protein 2

---

Tested Applications

• Positive WB detected in: A2780 cells, HEK-293 cells, HepG2 cells, Ramos cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00047 targets TORC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CREB regulated transcription coactivator 2
• Calculated Molecular Weight: 693 aa, 73 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC053562
• Gene Symbol: TORC2
• Gene ID (NCBI): 200186
• RRID: AB_3750099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q53ET0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .