TP63 Recombinant antibody

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  WB analysis using 86154-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-1-RR (TP63 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 86154-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86154-1-RR (TP63 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86154-1-RR against Human TP63 were performed. The affinity constant is below 1 pM.

TP63 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 86154-1-RR

Clone No.250042G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

p63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63

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Tested Applications

• Positive WB detected in: A431 cells, HaCaT cells, mouse skin tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86154-1-RR targets TP63 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TP63 fusion protein Ag2791
• Full Name: tumor protein p63
• Calculated Molecular Weight: 680 aa, 77 kDa
• Observed Molecular Weight: 63-68 kDa
• GenBank Accession Number: BC039815
• Gene Symbol: TP63
• Gene ID (NCBI): 8626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H3D4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).