hD53; TPD52L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

hD53; TPD52L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14732-1-AP

Synonyms

TPD53, TPD52L1, Tumor protein D52-like 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 14732-1-AP

  WB result of hD53; TPD52L1 antibody (14732-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-hD53; TPD52L1 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 14732-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14732-1-AP

  IP result of anti-hD53; TPD52L1 (IP:14732-1-AP, 3ug; Detection:14732-1-AP 1:300) with MCF-7 cells lysate 2500ug.

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  IHC staining of human prostate cancer using 14732-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14732-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

14732-1-AP targets hD53; TPD52L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: hD53; TPD52L1 fusion protein Ag6493
• Full Name: tumor protein D52-like 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC002375
• Gene Symbol: TPD52L1
• Gene ID (NCBI): 7164
• RRID: AB_2272159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tumor protein D52-like 1 (TPD52L1) gene, also known as hD53, encodes a member of the tumor protein D52 (TPD52) family. TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signaling intermediates and regulators of vesicle trafficking. hD53 may form homo- or heterodimers with other TPD52 family members, and it is reported to be involved in cell proliferation and calcium signaling. Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.

Publications

Species	Application	Title
human	WB	Cell Biosci; Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.; Authors - Yuzo Abe
human	IHC	Front Oncol; Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer.; Authors - Qin Hong
human,mouse	WB,IHC,IP	Int J Oncol; Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.; Authors - Kosuke Kato
	WB	Biochem J; Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability.; Authors - Hiromi Motohashi
		Sci Data; The Signaling Pathways Project, an integrated 'omics knowledgebase for mammalian cellular signaling pathways.; Authors - Scott A Ochsner