TPP1 Polyclonal antibody

TPP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12479-1-AP

Synonyms

CLN2, GIG1, LPIC, TPP 1, TPP I, TPP1, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 1, tripeptidyl peptidase I, UNQ267/PRO304

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Validation Data Gallery

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  WB analysis of human colon using 12479-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12479-1-AP (TPP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12479-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 12479-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 12479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12479-1-AP (TPP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12479-1-AP (TPP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12479-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12479-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12479-1-AP (TPP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human colon tissue
• Positive IHC detected in: human colon cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12479-1-AP targets TPP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPP1 fusion protein Ag3173
• Full Name: tripeptidyl peptidase I
• Calculated Molecular Weight: 563 aa, 61 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC014863
• Gene Symbol: TPP1
• Gene ID (NCBI): 1200
• RRID: AB_2205902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIPEPTIDYL PEPTIDASE I, TPP1, is a lysosomal exopeptidase that sequentially removes tripeptides from the N termini of proteins. The proenzyme has an apparent molecular mass of 66 kD, and the mature enzyme has an apparent molecular mass of 46 to 48 kD. Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2). This protein distributes widely in all tissues.

Publications

Species	Application	Title
mouse	WB	Mol Reprod Dev; Protein profiling the differences between diabetic and normal mouse cumulus cells.; Authors - Guang-Jian Jiang