Tested Applications
| Positive WB detected in | human colon tissue |
| Positive IHC detected in | human colon cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12479-1-AP targets TPP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3173 Product name: Recombinant human TPP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 215-563 aa of BC014863 Sequence: DVGSGTSNNSQACAQFLEQYFHDSDLAQFMRLFGGNFAHQASVARVVGQQGRGRAGIEASLDVQYLMSAGANISTWVYSSPGRHEGQEPFLQWLMLLSNESALPHVHTVSYGDDEDSLSSAYIQRVNTELMKAAARGLTLLFASGDSGAGCWSVSGRHEFRPTFPASSPYVTTVGGTSFQEPFLITNEIVDYISGGGFSNVFPRPSYQEEAVTKFLSSSPHLPPSSYFNASGRAYPDVAALSDGYWVVSNRVPIPWVSGTSASTPVFGGILSLINEHRILSGRPPLGFLNPRLYQQHGAGLFDVTRGCHESCLDEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLLNP Predict reactive species |
| Full Name | tripeptidyl peptidase I |
| Calculated Molecular Weight | 563 aa, 61 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC014863 |
| Gene Symbol | TPP1 |
| Gene ID (NCBI) | 1200 |
| RRID | AB_2205902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14773 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIPEPTIDYL PEPTIDASE I, TPP1, is a lysosomal exopeptidase that sequentially removes tripeptides from the N termini of proteins. The proenzyme has an apparent molecular mass of 66 kD, and the mature enzyme has an apparent molecular mass of 46 to 48 kD. Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2). This protein distributes widely in all tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TPP1 antibody 12479-1-AP | Download protocol |
| WB protocol for TPP1 antibody 12479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TPP1 Polyclonal antibody (Cat# 12479-1-AP) has a total of 2 citations. One citation appears in Oncology Reports, studying RFX5 as a transcriptional activator of TPP1 in hepatocellular carcinoma using IHC. The other is published in Molecular Reproduction and Development, profiling protein differences between diabetic and normal mouse cumulus cells using WB. The associated research fields are oncology and reproductive biology.
| Species | Application | Title |
|---|---|---|
Oncol Rep The transcription factor RFX5 is a transcriptional activator of the TPP1 gene in hepatocellular carcinoma. | ||
Mol Reprod Dev Protein profiling the differences between diabetic and normal mouse cumulus cells. |
