TPST1 Polyclonal antibody, PBS Only

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• TPST1 Polyclonal antibody, PBS Only (12404-1-PBS)

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  WB analysis using 12404-1-AP (same clone as 12404-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12404-1-AP (TPST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12404-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12404-1-AP (same clone as 12404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12404-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12404-1-AP (same clone as 12404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12404-1-PBS in a different storage buffer formulation.

TPST1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12404-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.8.2.20, TPST-1

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Product Information

12404-1-PBS targets TPST1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3093

  Product name: Recombinant human TPST1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-371 aa of BC013188

  Sequence: ECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE

• Full Name: tyrosylprotein sulfotransferase 1
• Calculated Molecular Weight: 370 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC013188
• Gene Symbol: TPST1
• Gene ID (NCBI): 8460
• RRID: AB_2877855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60507
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TPST1 is a human tyrosylprotein sulfotransferase that uses 3′phosphoadenosine-5′phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. High TPST1 expression was linked with clinicopathological characteristics and unfavorable prognosis of HNSC patients (PMID: 16859706). In lung cancer, TPST1 expression was associated with the tumor-node metastasis (TNM) stage and lymph node metastasis, suggesting that it may be a useful prognostic indicator for lung cancer patients (PMID: 18243191, 17028309).