TPTE2 Polyclonal antibody

TPTE2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25120-1-AP

Synonyms

Lipid phosphatase TPIP, TPIP, TPTE2

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Validation Data Gallery

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  WB analysis of L02 using 25120-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 25120-1-AP (TPTE2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 25120-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25120-1-AP (TPTE2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25120-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25120-1-AP (TPTE2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of L02 using 25120-1-AP

  Immunofluorescent analysis of L02 cells using 25120-1-AP (TPTE2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25120-1-AP is TPTE2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPTE2 fusion protein Ag19169
• Full Name: transmembrane phosphoinositide 3-phosphatase and tensin homolog 2
• Calculated Molecular Weight: 522 aa, 61 kDa
• Observed Molecular Weight: 38-60 kDa
• GenBank Accession Number: BC128146
• Gene Symbol: TPTE2
• Gene ID (NCBI): 93492
• RRID: AB_2879908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.