TRADD Polyclonal antibody

TRADD Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15468-1-AP

Synonyms

Hs.89862, TRADD

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Validation Data Gallery

• 

  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 15468-1-AP

  IP result of anti-TRADD (IP:15468-1-AP, 4ug; Detection:15468-1-AP 1:500) with Jurkat cells lysate 4000 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15468-1-AP targets TRADD in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRADD fusion protein Ag7768
• Full Name: TNFRSF1A-associated via death domain
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC004491
• Gene Symbol: TRADD
• Gene ID (NCBI): 8717
• RRID: AB_2209035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	WB	Free Radic Biol Med; TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis; Authors - Jiping Yao
mouse	WB,IF,IP	Free Radic Biol Med; Kv7.4 channel is a key regulator of vascular inflammation and remodeling in neointimal hyperplasia and abdominal aortic aneurysms.; Authors - Xi-Zhenzi Fan
mouse	WB	Int J Biol Sci; Mannan-Binding Lectin Deficiency Limits Inflammation-induced Myeloid-Derived Suppressor Cells Expansion via Modulating Tumor Necrosis Factor Alpha-triggered Apoptosis.; Authors - Lijun Dong
mouse	WB	Mol Med; Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis.; Authors - Tian-Ping Chen