TRADD Polyclonal antibody

TRADD Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15468-1-AP

Synonyms

Hs.89862, TRADD

---

Validation Data Gallery

• 

  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 15468-1-AP

  IP result of anti-TRADD (IP:15468-1-AP, 4ug; Detection:15468-1-AP 1:500) with Jurkat cells lysate 4000 ug.

---

Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15468-1-AP targets TRADD in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRADD fusion protein Ag7768
• Full Name: TNFRSF1A-associated via death domain
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC004491
• Gene symbol: TRADD
• Gene ID (NCBI): 8717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Mol Med; Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis.; Authors - Tian-Ping Chen ; KD Validated
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang