TRAF3IP3 Polyclonal antibody, PBS Only

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• TRAF3IP3 Polyclonal antibody, PBS Only (18110-1-PBS)

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  WB analysis of Jurkat using 18110-1-AP (same clone as 18110-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 18110-1-AP (same clone as 18110-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

TRAF3IP3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 18110-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

18110-1-PBS targets TRAF3IP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12559

  Product name: Recombinant human TRAF3IP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC110302

  Sequence: MISPDPRPSPGLARWAESYEAKCERRQEIRESRRCRPNVTTCRQVGKTLRIQQREQLQRARLQQFFRRRNLELEEKGKAQHPQAREQGPSRRPGQVTVLKEPLSCARRISSPREQVTGTSSEVFPAQHPPPSGICRDLSDHLSSQAGGLPPQDTPIKKPPKHHRGTQTKAEGPTIKNDASQQTNYGVAVLDKEIIQLSDYLKEALQRELVLKQKMVILQDLLSTLIQASDSSWKGQLNEDKLKGKLRSLENQLYTCTQKYSPWGMKKVLLEMEDQKNSYEQKAKESLQKVLEEKMNAEQQLQSTQRSLALAEQKCEEWRSQYEALKEDWRTLGTQHRELESQLHVLQSKLQIN

• Full Name: TRAF3 interacting protein 3
• Calculated Molecular Weight: 353aa,41 kDa; 551aa,64 kDa
• Observed Molecular Weight: 64 kDa, 47 kDa, 41 kDa
• GenBank Accession Number: BC110302
• Gene Symbol: TRAF3IP3
• Gene ID (NCBI): 80342
• RRID: AB_2918057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y228
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.