TRAF4 Monoclonal antibody

• Featured Product
• KD/KO Validated

TRAF4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2C9A11

Cat no : 66755-1-Ig

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

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Validation Data Gallery

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  WB analysis using 66755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66755-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66755-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66755-1-Ig (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66755-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66755-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66755-1-Ig (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, THP-1 cells, U-937 cells, NCCIT cells, human brain tissue, pig brain tissue, HEK-293 cells, Ramos cells, Daudi cells, Raji cells, pig spleen tissue
• Positive IHC detected in: human urothelial carcinoma tissue, human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

66755-1-Ig targets TRAF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAF4 fusion protein Ag27221
• Full Name: TNF receptor-associated factor 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC001769
• Gene Symbol: TRAF4
• Gene ID (NCBI): 9618
• RRID: AB_2882101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.

Publications

Species	Application	Title
human	WB,IF	Int J Mol Sci; TRAF4 Promotes the Proliferation of Glioblastoma by Stabilizing SETDB1 to Activate the AKT Pathway; Authors - Hongyu Gu ; KD Validated
mouse	WB	Anticancer Drugs; High expression of TRAF4 predicts poor prognosis in tamoxifen-treated breast cancer and promotes tamoxifen resistance.; Authors - Jun Zhou ; KD Validated
human	WB,IHC,IF	Adv Sci (Weinh); TRAF4-Mediated LAMTOR1 Ubiquitination Promotes mTORC1 Activation and Inhibits the Inflammation-Induced Colorectal Cancer Progression; Authors - Linlin Zhao ; KD Validated
human	WB	FEBS J; TRAF2 associates with cullin neddylation complex assembly; Authors - Tiantian Wang