TRAF6 Recombinant monoclonal antibody

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• TRAF6 Recombinant monoclonal antibody (83535-2-RR)

TRAF6 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83535-2-RR Copied!

Clone No. 230430H3

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC (Intra), ELISA

Synonyms

230430H3, E3 ubiquitin-protein ligase TRAF6, EC:2.3.2.27, Interleukin-1 signal transducer, RING-type E3 ubiquitin transferase TRAF6

• 

  WB analysis using 83535-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83535-2-RR (TRAF6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Jurkat using 83535-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug TRAF6 Recombinant antibody (83535-2-RR, Clone:230430H3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293T cells, HeLa cells, Jurkat cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83535-2-RR targets TRAF6 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag14858

  Product name: Recombinant human TRAF6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 221-522 aa of BC031052

  Sequence: ILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV

• Full Name: TNF receptor-associated factor 6
• Calculated Molecular Weight: 522 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC031052
• Gene Symbol: TRAF6
• Gene ID (NCBI): 7189
• RRID: AB_3743482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4K3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Publications

What published studies show

TRAF6 Recombinant monoclonal antibody (230430H3), catalog number 83535-2-RR, has 10 total citations. Citations appear in journals including J Nanobiotechnology, Cell Reports in Medicine, Journal of Translational Medicine, Free Radical Biology and Medicine, Journal of Ethnopharmacology, Frontiers in Endocrinology, International Immunopharmacology, Viro Sinica, Neurochemical Research, and Food Research International. Research fields span renal fibrosis, diabetic kidney disease, cancer radioresistance, bone loss, asthma, sepsis-induced liver injury, viral infection, neuroinflammation, and intestinal colitis.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Nanobiotechnology; M2 Macrophage membrane-mediated biomimetic nanoparticles carrying ADAM9 siRNA alleviate renal inflammation and fibrosis via the AKT/NF-κB pathway.; Authors - Qingxin Li
human,mouse	WB	Cell Rep Med; Phosphoglycerate kinase 1 contributes to diabetic kidney disease through enzyme-dependent and independent manners.; Authors - Hai-Jian Sun
		J Transl Med; Activation of NF-κB signaling pathway in GSDME-low esophageal squamous cell carcinoma cells enhances radioresistance.; Authors - Lingli Lei
mouse	WB	Free Radic Biol Med; Corosolic acid modulates RANKL-triggered signaling to suppress osteoclastogenesis and ovariectomy-induced bone loss.; Authors - Chuanyong Zhao
mouse	WB	J Ethnopharmacol; Gingerenone A ameliorates airway inflammation and remodeling in asthma by modulating the TLR4/MyD88/NF-κB pathway.; Authors - Yingjie Zhu
human	WB	Front Endocrinol (Lausanne); Inhibition of CYP450 family 1 subfamily B member 1 (CYP1B1) expression in macrophage reduces the inflammatory response in type 2 diabetes mellitus combined with tuberculosis; Authors - Qianqian Du