FcZero-rAb® CoraLite® Plus 488 Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 488 Anti-Human TRAIL/CD253 Rabbit Recombinant Antibody (CL488-FcA98139)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

TRAIL/CD253 FcZero-rAb^® Recombinant Antibody for FC

Cat No. CL488-FcA98139

Clone No.241419D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL

• 

  FC experiment of human PBMCs using CL488-FcA98139

  1x10^6 untreated (left) or IFN-a treated (right) human PBMCs were surface stained with FcZero-rAb® CoraLite® Plus 405 Anti-Human CD14 and 5 ul CoraLite® Plus 488 Anti-Human TRAIL/CD253 Rabbit RecAb (CL488-FcA98139, Clone: 241419D2). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL488-FcA98139

  1x10^6 IFN-a treated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human TRAIL/CD253 Rabbit RecAb (CL488-FcA98139, Clone: 241419D2) (red) or FcZero-rAb® CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9)(blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human TRAIL/CD253 Rabbit RecAb (CL488-FcA98139, Clone: 241419D2) (black,dashed). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: IFN-a treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-FcA98139 targets TRAIL/CD253 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50591
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.