TRAM1 Polyclonal antibody

TRAM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12705-1-AP

Synonyms

Translocating chain-associated membrane protein 1, TRAM, Protein TRAM1

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Validation Data Gallery

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  WB analysis of K-562 using 12705-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12705-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12705-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12705-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12705-1-AP (TRAM1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of PC-3 using 12705-1-AP

  Immunofluorescent analysis of PC-3 cells using 12705-1-AP (TRAM1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, Jurkat cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12705-1-AP targets TRAM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAM1 fusion protein Ag3384
• Full Name: translocation associated membrane protein 1
• Calculated Molecular Weight: 374 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC037738
• Gene Symbol: TRAM1
• Gene ID (NCBI): 23471
• RRID: AB_2282651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway; Authors - Sairong Fan
rat	WB	Evid Based Complement Alternat Med; The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota.; Authors - Boxun Zhang
human	WB	Mol Cell; An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins; Authors - Jia Ji